What is FLAER?

By William Aird

Fluorescent aerolysin, used in flow cytometry to diagnose paroxysmal nocturnal hemoglobinuria (PNH) (selectively binds the GPI anchor with high affinity).

Flow cytometric analysis of granulocytes using FLAER. (A) As a control setting, there was no evidence of decreased (type II PNH cells) or absent (type III PNH cells) expression of FLAER. (B) In this patient, decreased (42.4%) and absent (57.0%) FLAER fractions were detected, suggesting that 99.4% of PNH granulocyte clones had a FLAER deficiency, which is consistent with classic PNH. Source.