What is osmotic gradient ektacytometry?

By William Aird

Monitors the extent of red blood cell (RBC) shape change (elongation index) within a range of osmotic gradient under known shear stress and is a measure of hydration status of RBCs. Used to diagnose RBC membrane disorders.

The osmoscan module of LoRRca MaxSis is performed by adding 250 uL of whole blood to 5 mL of iso-osmolar polyvinylpyrrolidone (isoPVP). The diluted RBC suspension is submitted to an increasing osmotic gradient (from 80 mOsmol/L to 500 mOsmol/L) under a constant shear stress of 30 mPa. From Llaudet-Planas et al.

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