Knowledge Check • The Blood Project

HbA1c arises from:

a

A posttranslational event

b

A transcriptional event

Which of the following statements is/are correct:

a

Formation of HbA1c is a spontaneous reaction

b

Formation of HbA1c is an irreversible reaction

c

Formation of HbA1c occurs via an enzymatic reaction

d

HbA1c measures glycemic control over the previous 3 month period

HbA1c is formed by attachment of glucose to:

a

Beta chain of hemoglobin

b

Alpha chain of hemoglobin

Name glycated hemoglobins other than HbA1c

Click for Answer

HbA1a
HbA1c

Which methods can be used to assay HbA1c:

a

HPLC

b

Electrophoresis

Though not typically used now.

c

Immunoassays

d

Boronate affinity chromatography

e

Centrifugation

Sort the conditions according to whether they typically increase or decrease the HbA1c levels:

Hypertriglyceridemia

Iron deficiency

Hyperbilirubinemia

Acute blood loss

Uremia

Asplenia

Hemolysis

B12 deficiency

Splenomegaly

Increased A1C levels

Decreased A1C levels

What effect do hemoglobinopathies have on HbA1c levels?

Click for Answer

Can increase or decrease levels depending on the type of mutation and the degree of hemolysis