Serotonin release assay (SRA) for heparin-induced thrombocytopenia (HIT)

Serotonin release assay (SRA)

Serotonin release assay begins with normal donor platelets that are incubated with radioactive 12C-serotonin. The platelets, which now contain 12C-serotonin, are then incubated with control buffer or patient serum or plasma and various concentrations of heparin. HIT serum (or plasma) causes platelet activation at therapeutic (0.1–0.3 IU/ml) heparin concentrations, but not in the presence of high (100 IU/ml) heparin concentrations (not shown).

SRA reviewed by Warkentin and colleagues.

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